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Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
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Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis

Published on: March 10, 2014

Alkaline phosphatase reporter transposon for identification of genes encoding secreted proteins in gram-positive

Carmela M Gibson1, Michael G Caparon

  • 1Department of Molecular Microbiology, University Medical Center, Washington University School of Medicine, 660 S. Euclid Ave., St. Louis, Missouri 63110-1093, USA.

Applied and Environmental Microbiology
|February 2, 2002
PubMed

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...