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Quantitation of DNA sequences in environmental PCR products by a multiplexed, bead-based method
1Physics Department, Loyola College in Maryland, 4501 N. Charles Street, Baltimore, MD 21210, USA.
Applied and Environmental Microbiology
|February 2, 2002
Abstract:
A first application of a multiplexed, bead-based method is described for determining the abundances of target sequences in an environmental PCR product. Target sequences as little as 0.3% of the total amount of DNA can be quantified. Tests were conducted on 16S ribosomal DNA sequences from microorganisms collected from contaminated groundwater.