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Updated: Jul 19, 2026

Generation of Human Alloantigen-specific T Cells from Peripheral Blood
Published on: November 21, 2014
A proteome database of human primary T helper cells
T A Nyman1, A Rosengren, S Syyrakki
1Centre for Biotechnology, University of Turku and Abo Akademi University, Finland. tuula.nyman@btk.utu.fi
Abstract:
We have established the first public database of human primary T helper cell proteome using two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption/ionization-time of flight-mass spectrometry. For the database, CD4+ human T cells were activated with anti-CD3+anti-CD28 antibodies and metabolically labeled with [35S]methionine for 24 h. Cells were lysed and proteins were separated by 2-DE. About 1500 protein spots were detected in the resulting 2-DE gels with silver staining, and 2000 spots with autoradiography. We have identified 91 proteins from the 2-DE gels using peptide mass fingerprinting, and annotated them to our database. The identified proteins are also linked to SWISS-PROTand NCBI protein databases. Our database is available via the Internet at http://www3.btk.utu.fi:8080/Genomics/Proteomics/Database.
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