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Updated: Jul 19, 2026
09:47
Generation of Human Alloantigen-specific T Cells from Peripheral Blood
Published on: November 21, 2014
A proteome database of human primary T helper cells
T A Nyman1, A Rosengren, S Syyrakki
1Centre for Biotechnology, University of Turku and Abo Akademi University, Finland. tuula.nyman@btk.utu.fi
Electrophoresis
|February 5, 2002
Summary
Researchers created the first public human T helper cell proteome database. This resource catalogs identified proteins, aiding future immunological and proteomic research.
Area of Science:
- Proteomics
- Immunology
- Human Cell Biology
Background:
- Understanding the human T helper cell proteome is crucial for immunology and disease research.
- Existing proteomic data for primary T helper cells is limited, hindering comprehensive analysis.
Purpose of the Study:
- To establish the first publicly accessible database of the human primary T helper cell proteome.
- To catalog identified proteins and link them to existing protein databases for further research.
Main Methods:
- Human CD4+ T cells were activated and metabolically labeled with [35S]methionine.
- Proteins were separated using two-dimensional electrophoresis (2-DE).
- Protein identification was performed using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and peptide mass fingerprinting.
Main Results:
- Approximately 1500 protein spots were detected by silver staining and 2000 by autoradiography on 2-DE gels.
- 91 proteins were identified and annotated.
- The identified proteins were linked to SWISS-PROT and NCBI protein databases.
Conclusions:
- The developed database represents a significant resource for the scientific community.
- This proteomic catalog provides a foundation for further studies on T helper cell function and regulation.
- The database is accessible online, facilitating broad scientific access and utilization.
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