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High-performance liquid chromatographic analysis of angiotensin II receptor antagonist valsartan using a liquid
Noriko Daneshtalab1, Richard Z Lewanczuk, Fakhreddin Jamali
1Faculty of Pharmacy and Pharmaceutical Sciences and Department of Physiology, University of Alberta, Edmonton, Canada.
Purpose:
To develop an easy assay for the quantitation of the angiotensin II receptor antagonist valsartan in human plasma using a liquid extraction procedure.
Method:
The method involves acid extraction from 1 ml human plasma with methyl-tert.-butyl ether followed by back-extraction into a basic medium. An isocratic HPLC equipped with reverse phase column and a fluorescence detector was used at room temperature.
Results:
The response to 10-2000 ng/ml valsartan was linear. In plasma of three human subjects given 160 mg valsartan orally, concentrations of 25-1540 ng/ml were observed.
Conclusion:
This convenient method is suitable for pharmacokinetic studies of valsartan.
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