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[Effect of various methods of tissue storage on split-skin viability at 4 degrees C]
Objective:
To study various tissues and organs storage solutions (liver, kidney and corneal) at 4 degrees C for the storage of skin and the viability of skins stored in these solutions at correspondent condition.
Method:
Five experiment groups investigated for storing porcine split-skin at 4 degrees C were MEM, UW, MEM plus CS, UW-R and MEM-R. A new assay, WST-1, was applied to evaluate skin viability at 1, 3, 5, 7, 10 and 14 day during storage respectively.
Result:
Skin viability can all be protected by these three solutions. The effect of UW solution was superior to MEM. UW-R and MEM-R were superior to MEM and UW.
Conclusion:
The method of UW-R which replaces medium routinely may be an optimal method of storing skin at 4 degrees C.
