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Quantification of human immunodeficiency virus type 1 proviral DNA by using TaqMan technology

Yuqi Zhao1, Min Yu, Johann W Miller

  • 1Children's Memorial Institute for Education and Research, Northwestern University, Chicago, Illinois 60614, USA. yzhao@northwestern.edu

Journal of Clinical Microbiology
|February 5, 2002
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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