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[Experimental study of the transferred keratinocytes transplanted on biomembrane]
Zhao-yang Dong1, Yu-lin Chen, Yeurgen Kopp
1Department of Burns and Plastic Surgery, Shanghai Corps Hospital, Chinese People's Armed Police Force, Shanghai, P. R. China 201103.
Objective:
To search an ideal carrier of transferred keratinocytes for transplantation.
Methods:
The transferred keratinocytes were seeded on the surfaces of the artificial dermis and the silicone membrane and cultured in vitro for 2 weeks. The growth of the keratinocytes was observed by microscope and scanning electron microscope.
Results:
The keratinocytes implanted on the artificial dermis began to rupture and died after 2 to 3 days. While the keratinocytes adhered well on the surface of silicone membrane with pseudopodia formation after 1 week under scanning electron microscope, and the cells kept normal morphological and proliferative properties 2 weeks later.
Conclusion:
The silicone membrane can be applied as an useful carrier for the keratinocytes transplantation.