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Experimentally determined sequence requirement of ACGT-containing abscisic acid response element
Tsukaho Hattori1, Masako Totsuka, Tokunori Hobo
1Center for Molecular Biology and Genetics, Mie University, Tsu, 514-8507 Japan. hattori@gene.mie-u.ac.jp
Plant & Cell Physiology
|February 6, 2002
Abstract:
The sequence requirement of the ACGT-containing abscisic acid response element (ABRE) was analyzed by systematically substituting the bases surrounding the ACGT-core of motif A, the principal ABRE of the rice gene, OSEM: This was done within the context of a 55-bp promoter fragment that minimally confers ABA-responsiveness to a heterologous promoter. Based on this analysis, the sequence requirement of the ACGT-containing ABRE was determined as ACGTG G/T C, which matched very well with the consensus derived from sequence comparison of ABA-responsive promoters.