Experimentally determined sequence requirement of ACGT-containing abscisic acid response element
Related Experiment Video
Updated: Oct 2, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Tsukaho Hattori1, Masako Totsuka, Tokunori Hobo
1Center for Molecular Biology and Genetics, Mie University, Tsu, 514-8507 Japan. hattori@gene.mie-u.ac.jp
Abstract:
The sequence requirement of the ACGT-containing abscisic acid response element (ABRE) was analyzed by systematically substituting the bases surrounding the ACGT-core of motif A, the principal ABRE of the rice gene, OSEM: This was done within the context of a 55-bp promoter fragment that minimally confers ABA-responsiveness to a heterologous promoter. Based on this analysis, the sequence requirement of the ACGT-containing ABRE was determined as ACGTG G/T C, which matched very well with the consensus derived from sequence comparison of ABA-responsive promoters.
Related Concept Videos
Cis-regulatory Sequences
Cell Specific Gene Expression
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Stringent Response in E. coli

