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Updated: Aug 12, 2026
An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis
Published on: September 12, 2019
[IL-12 gene treatment of hepatocellular carcinoma: experimental study]
1Tumor Immunology and Gene Therapy Center, Eastern Institute of Hepatobiliary Surgery, Shanghai 200438.
Objective:
To investigate the inhibitory effects of retrovirus vector containing IL-12 gene on hepatoma growth in vivo and to explore a new approach of gene therapy to hepatocellular carcinoma (HCC).
Methods:
Retrovirus vector containing IL-12 gene was constructed and transfected into packaged cell PA317. Positive PA317 was injected into the rat which suffered from experimental HCC and its anti-tumor effects and immunity changes were recorded.
Results:
The packaged cell PA317 containing IL-12 gene could inhibit the proliferation of hepatocellular cell line CBRH3. The rats injected at day 1 or 3 can survive permanently, while those injected at day 5 or 7 can survive longer than those not injected. However, tumor generated in rats injected with blank control or package cells containing retroviral vector without IL-12 gene (P < 0.01).
Conclusions:
Package cells transfected with retroviral vector containing IL-12 either injected to the hepatoma tissue locally or given splenic exercises anti-hepatoma effects efficiently. The direct intrasplenic injection route is new, safe and effective.
