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Updated: Jul 28, 2026
A 3D System for Culturing Human Articular Chondrocytes in Synovial Fluid
Published on: January 31, 2012
[Transfection of articular chondrocytes with PcNDA3-hBMP3 and its stable expression]
Objective:
To explore the possibility of stable expression of PcDNA3-hBMP3 in cultured articular chondrocytes of rabbit.
Methods:
PcDNA3-hBMP3 was constructed using gene clone technique and recombined DNA technique. With the help of profectamine, the cultured articular chondrocytes were transfected with PcDNA3-hBMP3, and the evidence of successfully stable transfection in these cells could be obtained by positive northern blot.
Results:
The cultured articular chondrocytes of rabbits seemed to be polygonal, and its logarithmic growth phase was 2 - 4 days after cell inoculation. The two fragments cut from PcDNA3-hBMP3 by EcoR I and Xba I represented 5.4 kb and 1.4 kb by electrophoresis, which were confirmed to be the carrier and the fragment inserted originally, indicating that the construction of PcDNA3-hBMP3 was successful. The RNA extracted from cultured chondrocytes was screened for 4 weeks by G418 hibrided with the fragment cut from hBMP3 positively.
Conclusions:
With the help of profectamine, the cultured articular chondrocytes can be transfected by recombined gene of PcDNA3-hBMP3 successfully, and their stable expression at 4 weeks after transfection is obtained.
