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[Detection of telomerase activity in prostate needle-biopsy samples]
Objective:
To detect telomerase activity inprostate needle-biopsy samples and its role in diagnosis and prognosis of prostate cancer.
Methods:
20 tissue samples of prostate cancer tissues and 16 samples of adjacent tissues were obtained by needle-biopsy guided by transrectal B ultrasonography. 16 samples of benign prostatic hyperplasia tissues were obtained by prostatectomy and were confirmed pathologically. Telomerase activity was revealed by modified PCR-based telomeric repeat amplication protocol (TRAP)-silver staining assay.
Results:
Telomerase activity was revealed in 18 cases of prostate cancer. In tissues adjacent to prostate cancer, telomerase activity was found in 7 of 11 cases of prostatic intraepithelial neoplasia (PIN), and in 2 of 5 cases of BPH. None of tissue samples from patients with BPH showed telomerase activity.
Conclusions:
Telomerase activity in prostate needle-biopsy samples might be an useful marker for detecting prostate cancer and biological malignancy.