Cloning and nucleotide sequence of a linear DNA plasmid from Xanthophyllomyces dendrorhous (Phaffia rhodozyma)

Extrachromosomal elements were found in a strain of X. dendrorhous, and were characterized as linear DNA forming two well defined groups, pPh1 with 3 high-copy-number molecules, pPh11 (6.9 kb), pPh12 (5.7), pPh13 (4.7), and pPh2 with 2 low-copy-number molecules, pPh21 (3.6 kb), pPh22 (3.0). A 4077 bp fragment from pPh13 was cloned in pUC18 (pDK1) and sequenced (accession no. AJ 278,424). Seven putative ORF and some possible regulator sequences were defined.