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Mrnp41 (Rae 1p) associates with microtubules in HeLa cells and in neurons

D Kraemer1, T Dresbach, D Drenckhahn

  • 1Medizinische Poliklinik, Julius-Maximilians Universität, Würzburg, Germany. d.kraemer@medizin.uni-wuerzburg.de

European Journal of Cell Biology
|February 8, 2002
PubMed

Insights

Messenger ribonucleoprotein 41 (Mrnp41) binds to microtubules, influencing its cellular distribution. An intact microtubule network is essential for Mrnp41 particle dispersion to the cell periphery.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cytoskeletal Dynamics

Background:

  • Mrnp41 (hRae1p) is a conserved protein involved in nuclear mRNA export.
  • It localizes to the nuclear pore complex, nuclear domains, and cytoplasmic particles.

Purpose of the Study:

  • To investigate the association of Mrnp41 with microtubules.
  • To determine the role of the microtubule system in Mrnp41 localization.

Main Methods:

  • Immunofluorescence microscopy in HeLa cells and cultured neurons.
  • Co-purification of Mrnp41 with microtubules during polymerization/depolymerization.
  • Phosphocellulose chromatography to separate microtubule-associated proteins (MAPs).

Main Results:

  • Cytoplasmic Mrnp41 distribution is sensitive to nocodazole, causing peripheral disappearance and nuclear concentration.
  • Mrnp41 colocalizes with microtubules and exhibits an MT-like distribution in neurons.
  • Mrnp41 co-purifies with microtubules and MAPs, indicating a direct association.

Conclusions:

  • Mrnp41 associates with microtubules.
  • An intact microtubule system is required for the peripheral dispersion of Mrnp41-containing particles.
  • Mrnp41's role in cell division may involve its microtubule-binding ability.

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