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Related Experiment Videos

Histamine production by Haemophilus somnus.

Kevin W Ruby1, Ronald W Griffith, Merlin L Kaeberle

  • 1US Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary Services, Center for Veterinary Biologics, Licensing and Policy Development, Ames, IA 50010, USA. kevin.w.ruby@usda.gov

Comparative Immunology, Microbiology and Infectious Diseases
|February 8, 2002
PubMed
Summary

Haemophilus somnus bacteria produce and secrete histamine. Higher CO2 levels, similar to those in the bronchial tree, enhance histamine production, potentially explaining post-vaccination reactions.

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Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Immunology

Background:

  • Haemophilus somnus is a significant pathogen in bovine respiratory disease.
  • Post-vaccination reactions with H. somnus bacterins are occasionally observed.
  • The role of bacterial metabolites in host immune responses requires further investigation.

Purpose of the Study:

  • To investigate the production and secretion of histamine by Haemophilus somnus isolates.
  • To determine the effect of varying carbon dioxide (CO2) concentrations on histamine levels.
  • To explore the potential link between H. somnus-derived histamine and observed clinical reactions.

Main Methods:

  • Cultivation of H. somnus isolates on blood agar plates.
  • Enzyme-linked immunosorbent assay (ELISA) to quantify cell-associated and secreted histamine.

Related Experiment Videos

  • Incubation of bacterial strains under different CO2 concentrations to mimic bronchial conditions.

    • Main Results:

    • All tested H. somnus isolates exhibited cell-associated histamine.
    • Histamine secretion into growth medium was observed in specific strains.
    • Elevated histamine concentrations were detected in a respiratory isolate, particularly under high CO2 conditions (15-25%).

    Conclusions:

    • Haemophilus somnus isolates possess the capability to produce and secrete histamine.
    • Bronchial CO2 concentrations may enhance histamine production by H. somnus.
    • H. somnus-derived histamine may contribute to understanding post-vaccination reactions and the pathogenesis of bovine respiratory disease.