Related Experiment Videos
Performance and scale-up of adsorptive membrane chromatography.
Mark A Teeters1, Thatcher W Root, Edwin N Lightfoot
1Department of Chemical Engineering, University of Wisconsin-Madison, 53706, USA.
Journal of Chromatography. A
|February 8, 2002
Summary
Pall Corporation
Area of Science:
- Chromatography
- Separation Science
- Bioprocessing
Background:
- Conventional chromatography methods face challenges with scalability and efficiency.
- Membrane chromatography offers potential advantages in bioseparation processes.
Purpose of the Study:
- To evaluate the separation efficiency and scalability of Pall Corporation's Mustang stacked membrane chromatographic devices.
- To compare the performance of conventional and reverse-flow operations.
Main Methods:
- Utilized 10-ml and 1-L Mustang stacked membrane devices.
- Employed tracer pulse analysis with varying molecular mass tracers (AMP, lysozyme, thyroglobulin).
- Conducted numerical simulations of macroscopic flow.
Main Results:
- Mustang devices demonstrated insensitivity to tracer size and flow rate, yielding sharper peaks than conventional bead packings.
- Reverse-flow operation consistently produced sharper peaks compared to conventional flow.
- Non-uniform header residence times were identified as a primary cause for peak differences, with less impact on the 1-L device.
Conclusions:
- Pall Corporation's Mustang stacked membrane devices show promising separation performance and scalability.
- Reverse-flow operation enhances chromatographic peak sharpness.
- The 1-L device offers conservative scale-up potential due to reduced header residence time issues.
