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Related Experiment Videos

Maintenance of column performance at scale.

Alan William1, Kathy Taylor, Kyril Dambuleff

  • 1Separations Technical Group, Amersham Pharmacia Biotech, Piscataway, NJ 08855, USA. alan.williams@amershambiosciences.com

Journal of Chromatography. A
|February 8, 2002
PubMed
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New pack-in-place methods for Q Sepharose Big Beads columns were successfully scaled up. This chromatography technique achieved near-theoretical performance limits in large-scale industrial columns.

Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Chromatography

Background:

  • Large-scale chromatography relies on efficient column packing for optimal performance.
  • Traditional packing methods can be challenging to scale and may not achieve ideal bed uniformity.

Purpose of the Study:

  • To develop and evaluate pack-in-place column packing methods for Q Sepharose Big Beads.
  • To assess the scalability of these methods from 40 cm to 200 cm inner diameter (I.D.) columns.
  • To characterize the performance and stability of packed beds at industrial scales.

Main Methods:

  • Development of pack-in-place technology for Q Sepharose Big Beads.
  • Packing of 40 cm I.D. and 200 cm I.D. Chromaflow columns using the developed methods.
  • Evaluation of packed bed efficiency (Hred) and asymmetry (Af) at various test velocities and sample volumes.

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Main Results:

  • The pack-in-place method was successfully scaled up from 40 cm to 200 cm I.D.
  • Packed bed performance approached theoretical limits (Hred =2, Af=1) at both scales.
  • The stability of the packed beds and media compatibility with the technology were confirmed.

Conclusions:

  • Pack-in-place technology is an effective and scalable method for industrial chromatography column packing.
  • The developed method ensures high column performance and bed stability at large scales.
  • This approach is suitable for Q Sepharose Big Beads and Chromaflow column systems.