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[Expression of transforming growth factor-beta 1 mRNA and osteopontin mRNA in UMR-106 cells under mechanical
1Department of Orthodontics, School of Stomatology, Beijing Medical University, Beijing 100081, China.
Objective:
To investigate the effects of the mechanical stretching on the expression of osteopontin (OPN) mRNA and transforming growth factor-beta 1 (TGF beta 1) mRNA in a rat osteosarcoma cell line (UMR-106), and to unveil the cellular mechanism of mechanical force-induced bone remodeling.
Methods:
An apparatus was designed and fabricated by which force was loaded onto the cultured cells in vitro. Digoxin-labeled cDNA probes were used for in situ hybridization in combination with image analysis technique to relatively quantify the intensities of the hybridization signals.
Results:
Various magnitudes and durations of the mechanical stretching did exert different influences on the intensities of the mRNAs' expression. Of all the combinations, the low tension/frequency group showed the most remarkable alteration of TGF-beta 1, as increased from 0.0899 to 0.1756 (P < 0.01). Some putative relations lay between the expressions of the OPN mRNA and the TGF-beta 1 mRNA.
Conclusion:
The mechanical stretching can inevitably influence the expression of OPN mRNA and TGF-beta 1 mRNA. The beneficial alteration obtained in using low tension/frequency action mode suggests and verifies the use of relatively constant and light force to move teeth in clinical orthodontics.
