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[Influence of androgen on Bcl-2 mRNA expression in BPH tissue]
1Department of Urology, People's Hospital, Beijing Medical University, Beijing 100044, China.
Objective:
To study the expression of Bcl-2 mRNA in BPH tissue and the influence of androgen.
Methods:
The circulating testosterone level of BPH patients and rats was decreased by administration of medroxyprogesterone and stilbestrol as well as castration. Using RNA dot blot hybridization technic, we detected the Bcl-2mRNA expression in human BPH tissue and rat prostate under different level of androgen.
Results:
The average dot IOD (integrated optical density, IOD) value ratio of the untreated patient group to the treated group was 1.71 (P < 0.01). There was an decrease of intensity of Bcl-2 mRNA expression in the latter group compared with the former group. The ratio of the normal rat prostates to the castrated rat prostates was 1.28 (P < 0.05). The Bcl-2 mRNA expression was decreased after castration.
Conclusions:
The expression of Bcl-2 plays a role in the development of BPH. The decrease of prostatic cell death is important in the BPH pathogenesis. Apart from promoting the proliferation of prostatic epithelia and stroma cells, androgen might prohibit apoptosis by increasing the expression of Bcl-2 in BPH development.
Insights
Androgen influences benign prostatic hyperplasia (BPH) by increasing Bcl-2 mRNA expression, which inhibits prostatic cell death. This suggests a key role for Bcl-2 in BPH development.
Area of Science:
- Molecular biology
- Endocrinology
- Urology
Context:
- Benign prostatic hyperplasia (BPH) is a common condition in aging men.
- The role of androgens in BPH pathogenesis is well-established, involving epithelial and stromal cell proliferation.
- Mechanisms beyond proliferation, such as altered apoptosis, may also contribute to BPH development.
Purpose:
- To investigate the expression of B-cell lymphoma 2 (Bcl-2) messenger RNA (mRNA) in benign prostatic hyperplasia (BPH) tissue.
- To determine the influence of androgen levels on Bcl-2 mRNA expression in BPH and normal prostate tissue.
Summary:
- Bcl-2 mRNA expression was significantly decreased in BPH tissues and rat prostates after androgen levels were reduced (via medication or castration).
- RNA dot blot hybridization revealed lower Bcl-2 mRNA levels in treated BPH patients and castrated rats compared to controls.
- The integrated optical density (IOD) ratio of Bcl-2 mRNA expression was lower in treated BPH patients (1.71) and castrated rats (1.28) versus untreated/normal groups.
Impact:
- Results indicate that Bcl-2 expression plays a crucial role in BPH development.
- Androgen may inhibit apoptosis by upregulating Bcl-2 expression, contributing to the decrease in prostatic cell death observed in BPH.
- Understanding this mechanism could lead to novel therapeutic strategies targeting androgen-mediated apoptosis inhibition in BPH.