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Published on: February 8, 2020
[Expression of Fas ligand in urogenital malignant cell lines and renal cell carcinoma]
1Urological Department, First Hospital, Beijing Medical University, Beijing 100034, China.
Objective:
To explore the expression of Fas ligand in urogenital malignant cell lines and renal cell carcinoma.
Methods:
With immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR), we detected the expression of Fas ligand (FasL) in 6 urogenital malignant cell lines, bladder carcinoma cell lines (T(24), EJ, BIU-87), renal cell carcinoma cell lines (GRC-1, RCC-949), prostatic carcinoma cell line (PC-3M), and 10 cases of renal cell carcinoma.
Results:
The expression of FasL was detected in 3 (BIU-87, RCC-949, GRC-1) of the 6 urogenital malignant cell lines with immunocytochemistry, and 4 (BIU-87, RCC-949, GRC-1, PC-3M) of 6 urogenital malignant cell lines with RT-PCR method. No expression of FasL was detected in bladder carcinoma cell lines T(24) and EJ. Eight of 10 renal cell carcinoma tissues positively expressed FasL mRNA.
Conclusions:
FasL can express in urogenital malignant cell lines and renal cell carcinoma.

