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[Mutation and expression of nm23-H1 and CD44 gene in prostate cancers]
1Department of Urology, General Hospital, Tianjin Medical University, Tianjin 300052, China.
Objective:
To study the role of nm23-H1 and CD44 gene in carcinogenesis and metastasis of prostate cancers.
Methods:
Expression of nm23-H1/NDPK and CD44 protein were analyzed in 32 cases of prostate cancers (Pca) and 12 control of benign prostate hyperplasia (BPH) paraffin embedded tissue by immunohistochemistry. Mutation and expression of their genes were detected by silver SSCP method, semiquantitative RT-PCR, and southern blot hybridization analysis respectively in 25 cases of fresh Pca and 15 control BPH tissue.
Results:
nm23-H1 gene point mutation was detected in 13.3% (2/15) of Pca. Compared with the control tissue, the expression of nm23-H1 gene was increased significantly on both mRNA and protein levels in the carcinoma tissue (P < 0.05). The expression of nm23-H1 protein was also increased significantly in Pca with metastasis. The expression of CD44 standard form (CD44s) protein was significantly reduced in most Pca compared with BPH tissue. CD44s mRNA was detected in all Pca and BPH tissue, whereas CD44 variant (CD44v) without CD44v6 was mainly detected in carcinoma tissue.
Conclusions:
The altered expression of CD44s and CD44v may co-regulate in the carcinogenesis and progression, whereas nm23-H1 gene may play a different role from CD44 in the progression of prostate cancer. CD44v6 may be a useful but not significant tool for detection of colon or bladder cancers.