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[Telomerase activity and cellular apoptosis of bladder cancers]
1Department of Urology, First Hospital of Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China.
Objective:
To explore the relationship between clinicobiological acting, telomerase activity and cellular apoptosis of bladder cancer.
Methods:
The intensity of telomerase activity and cellular apoptosis of bladder cancer were detected by telomeric repeat amplification protocol (TRAP) and terminal deoxynucleotidyl-transferase mediated dUTP-biotin nick end labeling (TUNEL) assay respectively.
Results:
The positive rate of telomerase activity of bladder cancer was 89.29% and the apoptosis index of bladder cancer was (45.16 +/- 14.43)%. The intensity of telomerase activity and apoptosis index of bladder cancer were not related to patient's age, sex, size or number of tumor (P > 0.05), but to the "grade", "stage" and prognosis of bladder cancer (P < 0.01). The higher the intensity of telomerase activity or less cellular apoptosis, the poor prognosis of differentiated or more advanced bladder cancer. The intensity of telomerase activity was negatively correlated with cellular apoptosis of bladder cancer (r = -0.69, P < 0.01).
Conclusions:
The intensity of telomerase activity and cellular apoptosis are correlated with the grade, stage and prognosis of bladder cancer. The detection of telomerase activity and cellular apoptosis are helpful in clinical analysis and evaluation of prognosis of bladder cancer.