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Effect of interleukin-18 on mouse core body temperature
Silvia Gatti1, Jennifer Beck, Giamila Fantuzzi
1Pharmaceutical Division, CNS Preclinical Research, F. Hoffmann-LaRoche, CH-4070 Basel, Switzerland. silvia.gatti@roche.com
Summary
Recombinant murine interleukin-18 (rmIL-18) does not cause fever in mice. However, rmIL-18 pretreatment significantly reduces fever induced by interleukin-1 beta (IL-1beta), suggesting an anti-pyrogenic role.
Area of Science:
- Immunology
- Physiology
Background:
- Interleukin-18 (IL-18) is a cytokine involved in immune responses.
- The pyrogenic properties of IL-18 have not been fully elucidated.
Purpose of the Study:
- To investigate the pyrogenic effects of recombinant murine IL-18 (rmIL-18) in C57BL/6 mice.
- To compare the effects of rmIL-18 with other pyrogenic agents like IL-1beta and lipopolysaccharide (LPS).
- To determine if rmIL-18 can modulate fever responses induced by other cytokines.
Main Methods:
- Telemetry system used to monitor core body temperature in mice.
- Intraperitoneal injection of rmIL-18, human IL-1beta, LPS, and recombinant murine interferon-gamma (rmIFN-gamma).
- Assessment of fever response and modulation of IL-1beta-induced fever by rmIL-18 pretreatment.
Main Results:
- rmIL-18 and rmIFN-gamma did not induce significant fever at tested doses.
- IL-1beta and LPS induced fever within one hour post-injection.
- rmIL-18 pretreatment significantly attenuated IL-1beta-induced fever but not LPS-induced fever.
- IFN-gamma did not appear to mediate the observed attenuation.
Conclusions:
- rmIL-18 is not pyrogenic when administered intraperitoneally in C57BL/6 mice.
- rmIL-18 pretreatment exhibits an anti-pyrogenic effect against IL-1beta-induced fever.
- The mechanism of IL-18's anti-pyrogenic action warrants further investigation.