Aminopeptidase N is involved in cell motility and angiogenesis: its clinical significance in human colon cancer
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Summary
This summary is machine-generated.Aminopeptidase N (APN)/CD13 is a cell surface protein involved in cell motility and angiogenesis. Its expression indicates a poorer prognosis in colon cancer patients, particularly those with lymph node-positive disease.
Area Of Science
- Molecular biology
- Cancer research
- Immunology
Background
- Cell motility and angiogenesis are critical in tumor progression.
- Cell surface proteins play a role in regulating these processes.
- Gastrointestinal tumors involve complex molecular systems.
Purpose Of The Study
- Investigate cell surface proteins controlling cell motility and angiogenesis.
- Identify specific cell surface molecules in gastrointestinal tumors.
- Determine the prognostic significance of identified molecules in colon cancer.
Main Methods
- Developed functional monoclonal antibodies to inhibit cell motility and angiogenesis.
- Used antibody MH8-11 to identify the target epitope.
- Analyzed protein sequence and identified it as Aminopeptidase N (APN)/CD13.
- Correlated APN/CD13 expression with tumor status and patient survival in colon cancer.
Main Results
- Monoclonal antibody MH8-11 inhibited cell motility and in vitro angiogenesis.
- The epitope recognized by MH8-11 is Aminopeptidase N (APN)/CD13.
- APN/CD13 expression correlated with tumor status (P=0.025).
- Patients with positive APN/CD13 expression had significantly lower disease-free (P=0.014) and overall survival (P=0.033) rates.
- In node-positive patients, negative APN/CD13 expression was associated with better survival.
Conclusions
- APN/CD13 is implicated in cell motility and angiogenesis.
- APN/CD13 expression serves as a potential biomarker for poor prognosis in colon cancer.
- This finding is particularly relevant for node-positive colon cancer patients.