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Anti-hepatoma activity of resveratrol in vitro
Zhong-Jie Sun1, Cheng-En Pan, Hong-Shan Liu
1Department of Hepatobiliary Surgery, First Hospital of Xi'an Jiaotong University Xi'an 710061, Shaanxi Province, China. szhjie@21cn.com
Aim:
To study the anti-tumor effect of resveratrol alone and the synergistic effects of resveratrol with 5-FU on the growth of H22 cells line in vitro.
Methods:
The number of cells was measured by MTT method the morphological changes of H22 cells were investigated under microscopy and electron microscopy examination.
Results:
Resveratrol inhibited the growth of hepatoma cells line H22 in a dose- and time-dependent manner, IC50 of the resveratrol on H22 cells was 6.57mg x L(-1),The synergistic anti-tumor effects of resveratrol with 5-FU increased to a greater extent than for H22 cells treated with 5-FU alone (70.2% vs 28.4%) P<0.05 .Under microscope and electron microscope, characteristics of apoptosis such as typical apoptotic bodies were commonly found in tumor cells in the drug-treated groups.
Conclusion:
Resveratrol can suppresses the growth of H22 cells in vitro,its anti-tumor activity may occur through the induction of apoptosis.
