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Signal transduction pathway of nitric oxide inducing PC12 cell death
1Department of Neurosurgery, Zhujiang Hospital, First Military Medical University, Guangzhou, 510282, China.
Objective:
To study signal transduction pathway of nitric oxide inducing death of PC12 cells.
Methods:
Cell survival rate was measured with MTT assay, and caspase-3 activity with caspase-3 assay kits after PC12 cells were incubated with sodium nitroprusside (SNP), caspase-3 inhibitor II plus SNP or p38 inhibitor-SB203580 plus SNP.
Results:
SNP induced death of PC12 cells in dose- and time-dependent manner and enhanced caspase-3 activity gradually. Both caspase-3 inhibitor II and SB203580 reduced cell death, but SB203580 reduced caspase-3 activity significantly.
Conclusions:
NO may induce death of PC12 cells through activation of p38 and caspase-3.