Related Experiment Videos
[The isolation and characterization of dentin phosphoprotein from human dentine]
1Beijing Stomatological Hospital, Capital University of Medical Sciences, Beijing 100050.
Objective:
To establish the method of isolation and characterization of dentin phosphoprotein (DPP) from human dentine.
Methods:
Dentin powder was placed in dialysis tube after demineralized to completion with 0.6 mol/L HCl, then isolated DPP through DEAE-Sepharose CL6B anion exchange chromatography. Its protein composition, organic phosphorus content, molecular weight and amino acid were analyzed.
Results:
DPP contained high organic phosphorus, 23.8% of aspartic residue and 19.1% serine residue; their molecular weight were 141, 000, 124, 000, 108, 000 respectively.
Conclusion:
DPP can be extracted from human dentine with 0.6 mol/L HCl demineralization. and DEAE-Sepharose CL6B anion exchange chromatography. It is a group of proteins which have a high content of organic phosphorus and is found to be rich in serine and aspartic acid residues.