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Impairment of non-specific immunity in patients under persistent vegetative state resulting from trauma
Objective:
To investigate the immune response in patients under persistent vegetative state (PVS) resulting from trauma.
Methods:
Peripheral blood monocytes were obtained from 12 PVS patients (Group PVS)and individuals in normal control group by density gradient centrifugation; the enzyme linked immunosorbent assay kit was then used to measure monocyte HLA-DR antigen expression on the surface of peripheral blood monocytes before and after monocyte activation with either IFN-gamma or LPS.
Results:
Compared with normal control group, the peripheral blood monocyte HLA-DR antigen expression in the Group PVC fell significantly in PV S patients (P<0.001); the level of HLA-DR antigen express ion on the monocyte surface rose notably after stimulation with either IFN-gamma or LPS (P<0.01). As for PVS patients, it was still difficult to restore to normal (P<0.05).
Conclusions:
The level of HLA-DR antigen expression on the sur face of peripheral blood monocytes in PVS patients decreases obviously. Therefore, the function of non-specific immunity in PVS patients is suppressed.