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[A study of interferon alpha-2b as an accessory drug after glaucoma filtration surgery]
1Department of Ophthalmology, Union Hospital, Tongji Medical University, Wuhan 430022.
Objective:
To elucidate the inhibitory action of interferon alpha-2b (IFN alpha-2b) on the cicatrization under conjunctiva and the best way to use it after glaucoma filtration surgery.
Methods:
Immunohistochemical PAP method and image biological system 2000 (IBAS 2000) were utilized to quantitatively analyze the inhibitory effect of IFN alpha-2b on the expression of epidermal growth factor receptor (EGFR) of cultured calf fibroblasts; the effects of IFN alpha-2b on collagen contents in suture-caused cicatrization under conjunctiva and changes of aqueous humor protein after trabeculectomy performed on rabbits have also been observed.
Results:
IFN alpha-2b reduced the expression of EGFR of cultured bovine fibroblasts at a dose-dependent manner. The relationship between the EGFR and the dosage of IFN alpha-2b is Y = 2953.8 - 554.5 lgx (r = -0.98, P < 0.01). With 4.67 x 10(-5) micromol/L IFN alpha-2b not only reduced about 50% of the collagen in suture-caused cicatrization, but also significantly decreased the protein concentration of aqueous humour 3 and 72 hours after trabeculectomy.
Conclusion:
It is promising that IFN alpha-2b can be used after glaucoma filtration surgery to reduce excessive cicatrization with many advantages compared with antimetabolites.