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An Orthotopic Murine Model of Human Prostate Cancer Metastasis
06:48

An Orthotopic Murine Model of Human Prostate Cancer Metastasis

Published on: September 18, 2013

Sulforaphane and its metabolite mediate growth arrest and apoptosis in human prostate cancer cells

J W Chiao1, F-L Chung, R Kancherla

  • 1Department of Medicine, New York Medical College, Valhalla, NY 10595, USA.

International Journal of Oncology
|February 12, 2002
PubMed

Insights

Sulforaphane (SFN) and its metabolite SFN-NAC from cruciferous vegetables induce prostate cancer cell death and growth arrest. These compounds show similar activity, suggesting SFN

Area of Science:

  • Oncology
  • Nutritional Science
  • Biochemistry

Background:

  • Reduced prostate cancer incidence is linked to cruciferous vegetable consumption.
  • The specific phytochemicals responsible for this effect remain unidentified.

Purpose of the Study:

  • To investigate the effects of sulforaphane (SFN) and its metabolite SFN-N-acetylcysteine conjugate (SFN-NAC) on prostate cancer cells.
  • To elucidate the mechanisms by which SFN and SFN-NAC influence prostate cancer cell growth and apoptosis.

Main Methods:

  • Utilized the androgen-dependent human prostate cancer LNCaP cell line model.
  • Assessed cell growth, apoptosis, androgen receptor and prostate specific antigen expression, DNA synthesis, cell cycle progression, DNA strand breaks, and caspase activation.

Main Results:

  • SFN and SFN-NAC demonstrated dose-dependent induction of apoptosis and growth arrest in prostate cancer cells.
  • Activated caspases and detected DNA strand breaks in apoptotic cells.
  • Attenuated androgen receptor and prostate specific antigen expression, inhibited cyclin D1 and DNA synthesis, and induced G1 cell cycle arrest.

Conclusions:

  • SFN and its metabolite SFN-NAC exhibit similar anti-cancer activities, indicating SFN's effects are maintained through metabolic processes.
  • SFN is a potent dietary component in cruciferous vegetables with significant potential in prostate cancer prevention.

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