Related Experiment Video
Updated: Oct 2, 2026

Intranasal Delivery of mRNA Polyplexes via Rayleigh Breakup Aerosols: An In Vitro Method for Nasal Deposition and Functional Testing
Published on: January 20, 2026
Validity of in vitro tests on aqueous spray pumps as surrogates for nasal deposition
Julie D Suman1, Beth L Laube, Ta-Chun Lin
1University of Maryland, Department of Pharmaceutical Sciences, Baltimore, Maryland 20201, USA. jsuma001@umaryland.edu
Purpose:
To determine whether deposition pattern is related to in vitro measurements of droplet size, plume geometry, and spray pattern between two different nasal spray pumps believed to have different performance characteristics.
Methods:
Ten healthy volunteers inhaled radiolabeled saline from two different spray pumps (pump A and pump B). Deposition pattern was quantified from lateral views of the nose by gamma scintigraphy, expressed as the ratio of anterior to posterior (I:O) and superior to inferior (U:L) deposition. Droplet size was determined by Malvern Mastersizer S. Spray patterns were determined at 2.5 and 5 cm from the tip of the spray nozzle. Two-dimensional images of the emitted plume were captured by high-speed still photography.
Results:
There were no significant differences in I:O or U:L ratios for pump A compared to pump B, indicating no significant differences in deposition pattern. The volume diameters, Dv10 and Dv50, were not statistically different for pump A compared to pump B. There was a significant difference in Dv90 between pump A and pump B, (86.9 +/- 5.8 microm and 77.4 +/- 2.4 microm. respectively; P < 0.001). The ratio of the longest to shortest diameter for the spray pattern with pump A was 1.26 +/- 0.06 at 2.5 cm and 1.44 +/- 0.08 at 5 cm. The ratio for pump B was 1.13 +/- 0.03 at 2.5 cm and 1.19 +/- 0.05 at 5 cm. Ratios at both heights were statistically different for pump A compared to pump B (P < 0.00002 and P < 0.000001, respectively) Plume geometry analysis indicated statistical differences in both the width (17.0 +/- 0.97 vs. 18.5 +/- 0.56 cm, respectively: p<0.001) and the maximum length of the plumes (46.0 +/- 1.83 vs. 53.1 +/- 4.88 cm, respectively; p < .002). The differences in velocity of the plume and spray angle between the two pumps were not statistically different. CONCLUSIONS. Certain in vitro tests detected performance differences between the two pumps. However, these differences did not translate into different deposition patterns in vivo.
More Related Videos
07:28Dry Powder and Nebulized Aerosol Inhalation of Pharmaceuticals Delivered to Mice Using a Nose-only Exposure System
Published on: April 6, 2017
05:07Development of a Nose-only Inhalation Toxicity Test Chamber That Provides Four Exposure Concentrations of Nano-sized Particles
Published on: March 18, 2019
Related Concept Videos
In Vitro Drug Dissolution: Compendial Testing Models II
In Vitro Drug Dissolution: Alternative Methods
In Vitro Drug Dissolution: Compendial Testing Models I
In Vitro Drug Release Testing: Overview, Development and Validation
Bioequivalence studies: Biowaivers