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Type 2 angiotensin II receptor expression in human renal allografts: an association with chronic allograft

B N Becker1, L M Jacobson, D A Hullett

  • 1Department of Medicine, University of Wisconsin, Department of Veterans Affairs Hospital, Madison 53705, USA. bnb@medicine.wisc.edu

Clinical Nephrology
|February 12, 2002
PubMed
Abstract

Insights

The type 2 angiotensin II receptor (AT2R) is expressed in adult human renal transplants. Its expression correlates with genes involved in extracellular matrix regulation and chronic allograft nephropathy.

Area of Science:

  • Nephrology
  • Immunology
  • Molecular Biology

Background:

  • The renin-angiotensin system (RAS) is implicated in renal fibrosis via the type I angiotensin II receptor (AT1R).
  • The role of the type 2 angiotensin II receptor (AT2R) in renal fibrosis in adult human tissue remains unclear.

Purpose of the Study:

  • To investigate the expression and role of AT2R in adult human renal allografts.
  • To determine if AT2R expression correlates with fibrotic processes and chronic rejection.

Main Methods:

  • Reverse-transcription polymerase chain reaction (RT-PCR) was used to measure AT2R mRNA in renal transplant biopsy samples.
  • Correlations between AT2R mRNA, matrix-modulating genes, and chronic rejection histology were assessed.

Main Results:

  • AT2R mRNA was detected and confirmed by sequence analysis.
  • AT2R mRNA expression positively correlated with angiotensinogen and matrix-modulating genes (TGF-beta, MMP-1, TIMP-2, TIMP-3).
  • AT2R mRNA and protein levels were higher in patients with chronic allograft nephropathy.

Conclusions:

  • AT2R mRNA is expressed in adult human renal transplants.
  • AT2R expression is associated with matrix-modulating genes, suggesting a role in extracellular matrix regulation.
  • These findings hypothesize a link between AT2R and the pathogenesis of chronic allograft nephropathy.

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