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Updated: Oct 2, 2026

Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Published on: July 28, 2023
[Response of keloid fibroblasts to the effect of tumor necrosis factor-alpha(TNF-alpha)]
1Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Objective:
To investigate the biologic behavior of keloid fibroblasts and the pathogenesis of keloid.
Methods:
Human dermal fibroblasts cultured from normal skin and keloid were treated with tumor necrosis factor-alpha(TNF-alpha) for 48 hours at 10(3) U/ml and 10(4) U/ml. Collagen production by fibroblasts was assessed by 3H-proline in corporation into pepsin-resistant, precipitated extracellular collagenous protein.
Results:
TNF-alpha caused a concentration-dependent reduction in collagen production in normal dermal fibroblasts. Treatment with TNF-alpha at 10(3) U/ml significantly reduced the collagen synthesis in keloid fibroblasts (P < 0.01). However, the collagen synthesis in keloid fibroblasts did not further decreased after exposure to TNF-alpha at 10(4) U/ml.
Conclusions:
Keloid fibroblasts may remain less sensitive to the inhibitory effects of TNF-alpha on collagen synthesis to a certain extent.
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