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[An experimental study of repairing nerve gap with acellular basal lamina tubes]
1Department of Plastic & Reconstructive Surgery of Shanghai Ninth People's Hospital, Tissue Engineering Center of Shanghai Second Medical University, Shanghai 200011, China.
Objective:
To develop an effective new material for repair of nerve defects.
Methods:
The 4-week pre-degenerative and normal sciatic nerve segments of SD rats were extracted with the detergent lysophosphatidylcholine, which resulted in acellular nerve basal lamina tubes, called EDNG (extracted degenerative nerve grafts) and ENG (extracted normal nerve grafts) respectively. The morphology of the acellular nerve basal lamina tubes was revealed by conventional histological examinations, transmission electron microscopy and immunohistochemistry method. The basal lamina tubes, as allografts, were used to repair a 15 mm-gap of sciatic nerve in Wistar rats. In the control group nerve autografts (NAG) was applied. Evaluation included general observation, electromyographic examination, muscle measurement and histological observation of serial sections at 12 weeks after surgery.
Results:
The EDNG had more ideal frame, better resilience than ENG. There was no any cell debris in EDNG, whereas in the inner wall of the tubes immunohistochemical analysis revealed clear positive staining and strong immunoreactivity of laminin under immunoelectron microscopy. After peripheral nerves were repaired, all the animals of the three groups had restoration of function to a certain extent. The result of the NAG group was the best, the EDNG group was the next and the ENG group was the inferior.
Conclusion:
This new material offers a possible solution to repair of a short defect of a peripheral nerve.