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[Single nucleotide polymorphism analysis in chicken insulin-like growth factor-II gene and its associations with
Bing-Xue Yan1, Ning Li, Xue-Mei Deng
1National Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100094, China.
Abstract:
In this experiment, F2 chicken derived from Broilers crossing with Silky was used to study the effect of insulin-like growth factor-II gene on growth and carcass traits. The partial gene was amplified by two pairs of primers, and single nucleotide polymorphism (SNPs) was detected by the technique of restriction fragment length polymorphism (RFLP), and then confirmed by DNA sequencing. The mutation was found in the exon-2 of the gene, and can be clarified by cutting of restriction enzyme Aci-I. The result of least square analysis showed the gene was significantly related with growth and carcass traits. It implied that the insulin-like growth factor-II gene could be a genetic locus or linked to a major gene affecting greatly the growth and carcass traits in chicken.
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