Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[PCR walking in cotton genome using YADE method].

Yue-Hua Xiao1, Ming Luo, Wei-Guo Fang

  • 1Biotechnology Research Center, Southwest Agricultural University, Chongqing 400716, China.

Yi Chuan Xue Bao = Acta Genetica Sinica
|February 13, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Experimental Detection and Simulation of Terahertz Spectra of Aqueous L-Arginine.

Biosensors·2022
Same author

Quantifying substantial carcinogenesis of genetic and environmental factors from measurement error in the number of stem cell divisions.

BMC cancer·2022
Same author

Association of cognitive function and hypoperfusion in Moyamoya disease patients without stroke.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism·2022
Same author

Insights into the Novel <i>FAD2</i> Gene Regulating Oleic Acid Accumulation in Peanut Seeds with Different Maturity.

Genes·2022
Same author

Quantitative analysis of aqueous biomolecular mixtures by THz spectroscopy based on high-power LiNbO<sub>3</sub> radiation source.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2022
Same author

Porous Se@SiO<sub>2</sub> nanoparticles improve oxidative injury to promote muscle regeneration via modulating mitochondria.

Nanomedicine (London, England)·2022

A novel Y-shaped adaptor dependent extension (YADE) method effectively amplifies unknown genomic DNA sequences adjacent to known DNA. This technique suppresses single-primer amplification, enabling precise gene discovery and analysis.

Area of Science:

  • Genomics
  • Molecular Biology
  • Biotechnology

Context:

  • Amplifying unknown genomic DNA sequences adjacent to known sequences is crucial for gene discovery.
  • Existing methods may suffer from limitations like single-primer amplification.
  • The cotton ovule cDNA fragment (F027) serves as a reference point for adjacent sequence identification.

Purpose:

  • To develop and validate a Y-shaped adaptor dependent extension (YADE) method for amplifying unknown genomic sequences.
  • To suppress single-primer amplification artifacts.
  • To identify and characterize genomic fragments adjacent to a known cotton ovule cDNA fragment.

Summary:

  • A Y-shaped adaptor dependent extension (YADE) method was developed, completely suppressing single-primer amplification.

Related Experiment Videos

  • Two genomic fragments, F027S and F027A, adjacent to a cotton ovule cDNA fragment (F027), were successfully amplified.
  • F027S and F027A showed significant overlap with F027 and contained putative introns with conserved spliceosomal border sequences (GT-AG) and branch sites.

    • Impact:

    • The YADE method offers a robust approach for exploring flanking genomic regions.
    • This technique enhances the accuracy of identifying novel gene structures and regulatory elements.
    • Potential improvements and diverse applications of the YADE method in genomic research are discussed.