[Molecular cloning of specific porcine adipocyte membrane protein cDNA]

Specific porcine adipocyte membrane protein (PAMP), which was recently reported, is probably associated with the growth and development of adipocyte and regulator of adipose tissue. Using RT-PCR and RACE methods, and primer designed according to published sequence of PAMP gene 5' end, we have successfully amplified about a 500 bp and a 400 bp from pig adipose tissue. A 500 bp fragment was retrieved, cloned and sequenced. The sequencing result showed that we have got the clone and sequence of cDNA 5'-end for PAMP gene.