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Related Experiment Videos

Salmonella-mediated mucosal cell-mediated immunity.

J W Lillard1, P N Boyaka, S Singh

  • 1Department of Microbiology, Biochemistry and Immunology, Morehouse School of Medicine, Atlanta, GA 30310-1495, USA. Lillard@msm.edu

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|February 13, 2002
PubMed
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Oral Salmonella vaccines expressing antigens induce strong mucosal IgA and Th1-type immune responses. This approach effectively generates cell-mediated immunity (CMI) and cytotoxic T lymphocytes (CTLs) against tumors.

Area of Science:

  • Immunology
  • Microbiology
  • Vaccinology

Background:

  • Recombinant Salmonella typhimurium strains expressing antigens can induce mucosal and systemic immune responses.
  • The role of Salmonella-induced Th1 cell phenotype in mucosal cell-mediated immunity (CMI) requires further elucidation.

Purpose of the Study:

  • To construct an attenuated Salmonella typhimurium strain expressing chicken egg albumin (OVA).
  • To investigate the role of Salmonella-induced Th1 cell phenotype on mucosal cell-mediated immunity (CMI).

Main Methods:

  • Construction of an attenuated Salmonella typhimurium (aroA- aroD-) strain expressing chicken egg albumin (OVA).
  • Oral immunization of mice with the Salmonella-OVA vaccine.
  • Analysis of Peyer's patches and spleen lymphocytes for cell lysis of the H-2b restricted EG7.OVA tumor cell line.

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Main Results:

  • A single dose of the oral Salmonella-OVA vaccine induced significant increases in cell lysis of the EG7.OVA tumor cell line.
  • The vaccine elicited systemic and mucosal Th1-type responses and cytotoxic T lymphocytes (CTLs).
  • Evidence supports a regulated relationship between cell-mediated immunity and mucosal IgA responses.

Conclusions:

  • Oral Salmonella vaccines expressing antigens are effective in eliciting Th1-type immune responses and CTLs.
  • A single dose of the recombinant Salmonella vaccine vector is sufficient to induce these responses.
  • The study supports the interplay between cell-mediated immunity and mucosal humoral immunity (IgA).