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Galectin-8 Expression Decreases In Cancer Compared With Normal And Dysplastic Human Colon Tissue And Acts Significantly On Human Colon Cancer Cell Migration As A Suppressor.

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Galectin-8 Expression Decreases In Cancer Compared With Normal And Dysplastic Human Colon Tissue And Acts Significantly On Human Colon Cancer Cell Migration As A Suppressor.

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Galectin-8 expression decreases in cancer compared with normal and dysplastic human colon tissue and acts

N Nagy¹, Y Bronckart, I Camby

¹Laboratory of AnatomoPathology, Erasmus University Hospital, Free University of Brussels (ULB), Brussels, Belgium.

|February 13, 2002

View abstract on PubMed

Summary

This summary is machine-generated.

Galectin-8 expression decreases with colon cancer progression and suppresses tumor growth. Its inhibitory effect on migration is observed in slower-growing colon cancer models, suggesting a potential therapeutic role.

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Area of Science:

Oncology
Molecular Biology
Biochemistry

Background:

Galectins, beta-galactoside binding proteins, influence cell adhesion and migration in human colon cancer.
Previous studies focused on galectins-1 and -3, leaving other family members, like galectin-8, under-investigated in colon tumorigenesis.

Purpose of the Study:

To quantitatively assess galectin-8 immunohistochemical expression in normal, benign, and malignant human colon tissues.
To investigate the role of galectin-8 in colon cancer cell lines (HCT-15, LoVo, CoLo201, DLD-1) and their in vivo xenografts.
To determine the effect of galectin-8 and its neutralizing antibody on colon cancer cell migration.

Main Methods:

Quantitative immunohistochemistry and glycohistochemistry for galectin-8 expression and sugar-binding ability.
Reverse transcriptase-polymerase chain reaction to detect galectin-8 mRNA.
Fluorescence microscopy to visualize exogenously added galectin-8 localization.
Computer-assisted microscopy to quantify cell migration in response to galectin-8.

Main Results:

Galectin-8 expression significantly decreased with increasing malignancy and invasion depth in human colon tissues.
In vitro, experimental colon cancer models showed higher galectin-8 staining than their in vivo counterparts.
Galectin-8 expression was inversely related to tumor growth rate in nude mouse xenografts.
In vitro, galectin-8 reduced migration only in the two models with the slowest in vivo growth rates.

Conclusions:

Galectin-8 expression correlates with colon cancer malignancy and exhibits suppressor activity.
The inhibitory effect of galectin-8 on cell migration is specific to colon cancer models with lower growth rates.
Further research should explore novel galectins beyond galectins-1 and -3 for their roles in cancer.