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Related Experiment Videos

NCAM regulates cell motility.

Søren Prag1, Eugene A Lepekhin, Kateryna Kolkova

  • 1Protein Laboratory, Institute of Molecular Pathology, University of Copenhagen, Denmark.

Journal of Cell Science
|February 13, 2002
PubMed
Summary
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Neural cell adhesion molecule (NCAM) regulates glioma cell motility. NCAM-140 expression reduces cell movement by affecting attachment and interacting with intracellular factors, while its extracellular portion inhibits migration via heparan sulfate proteoglycans.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • Cell migration is crucial for nervous system development.
  • Regulatory mechanisms governing cell motility are not fully understood.
  • Neural cell adhesion molecule (NCAM) is implicated in cell behavior.

Purpose of the Study:

  • To investigate the role of NCAM in regulating glioma cell motility.
  • To elucidate the specific mechanisms by which NCAM isoforms affect cell migration.
  • To identify potential NCAM interactions involved in motility regulation.

Main Methods:

  • Examining the effect of NCAM expression and exposure on glioma cell motility.
  • Analyzing cell attachment to fibronectin in NCAM-expressing versus NCAM-negative cells.

Related Experiment Videos

  • Investigating the role of NCAM intracellular and extracellular domains using ectopic expression and interaction studies.

    • Main Results:

    • NCAM expression significantly reduced glioma cell motility, independent of homophilic interactions.
    • NCAM-140 isoform expression decreased cellular attachment to fibronectin.
    • The cytoplasmic part of NCAM-140 inhibited motility, likely via interaction with p59(fyn).
    • The extracellular part of NCAM acted as a paracrine inhibitor, interacting with heparan sulfate proteoglycans.

    Conclusions:

    • NCAM plays a dual role in regulating glioma cell motility through both intracellular and extracellular mechanisms.
    • NCAM-140 influences cell adhesion and motility through direct interactions.
    • Extracellular NCAM inhibits cell locomotion via heterophilic interactions with cell-surface proteoglycans.