Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Geldanamycin specifically modulates thrombin-mediated morphological changes in mouse neuroblasts.

Karnire Sadashiva Pai1, Dennis D Cunningham

  • 1Department of Microbiology & Molecular Genetics, University of California, Irvine, California 92697-4025, USA.

Journal of Neurochemistry
|February 14, 2002
PubMed
Summary

Geldanamycin, an Hsp90 inhibitor, prevents thrombin-induced neuronal retraction by blocking RhoA activation. This suggests geldanamycin specifically regulates thrombin

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

Etv5 Is Required for Proper Maturation and Dendritic Development of Adult-Born Granule Cells in the Dentate Gyrus.

Journal of neurochemistry·2026
Same journal

Preface to the Special Issue: "Molecular and Cellular Mechanisms of Memory".

Journal of neurochemistry·2026
Same journal

Rethinking Alzheimer's Disease Therapy: From Amyloid-Centric Approaches to Multi-Target Phytochemical Strategies.

Journal of neurochemistry·2026
Same journal

Caffeine Regulates GABA Transport Homeostasis in the Adolescent Mouse Frontal Cortex via Adenosine A1 Receptor and PKC-Dependent Pathways.

Journal of neurochemistry·2026
Same journal

PBMC circRNA Profiles Distinguish Atypical From Idiopathic Parkinsonism and Track Clinical Severity Across Syndromes.

Journal of neurochemistry·2026
Same journal

From Synapses to Circuits, the Role of KIBRA and the WWC Family in Adaptive Brain Function.

Journal of neurochemistry·2026

Area of Science:

  • Neuroscience
  • Cell Biology
  • Pharmacology

Background:

  • Neuronal morphology and process extension are crucial for synaptic function.
  • Thrombin, a serine protease, regulates neuronal morphology via protease-activated receptor-1 (PAR-1).
  • Modulating thrombin-induced morphological changes has therapeutic potential.

Purpose of the Study:

  • To investigate the role of geldanamycin, a heat shock protein 90 (Hsp90) inhibitor, in thrombin-mediated neuronal morphology regulation.
  • To determine if geldanamycin affects thrombin-induced RhoA activation.
  • To assess the specificity of geldanamycin's effects on thrombin-induced versus lysophosphatidic acid (LPA)-induced changes.

Main Methods:

  • Mouse neuroblasts (NB2a) were treated with varying concentrations of geldanamycin and thrombin.

Related Experiment Videos

  • Neurite retraction was measured to assess morphological changes.
  • RhoA activation was analyzed following thrombin or LPA stimulation in the presence or absence of geldanamycin.

    • Main Results:

    • Geldanamycin prevented thrombin-induced neurite retraction in a dose-dependent manner.
    • Geldanamycin inhibited thrombin-induced activation of RhoA, a key cytoskeletal regulator.
    • Geldanamycin did not affect LPA-induced neurite retraction or RhoA activation, indicating specificity.

    Conclusions:

    • Geldanamycin specifically inhibits thrombin-induced neuronal morphological changes.
    • The mechanism involves the blockade of thrombin-induced RhoA activation.
    • Geldanamycin shows potential for therapeutic applications in conditions involving thrombin-mediated neuronal alterations.