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Matrix and serine protease expression during leukemic cell differentiation induced by aclacinomycin and
Laetitia Devy1, Patrick Hollender, Carine Munaut
1Laboratoire de Biologie des Tumeurs et du Développement, Université de Liège, Sart-Tilman, Belgium.
Abstract:
In myeloid leukemia, immature leukemic cells are able to egress into peripheral blood to infiltrate extra-medullary organs. We therefore analyzed the migrating and invasive potential of human HL-60 and NB4 cell lines, representative of acute myelogenous leukemia, their ability to express matrix metalloproteases (MMPs), tissue inhibitors of metalloproteases (TIMPs) and urokinase plasminogen activator (uPA) in response to differentiating agents. Granulocytic differentiation by all-trans-retinoic acid (ATRA) and aclacinomycin (ACLA) strongly increased HL-60 and NB4 cell migration and invasion. At mRNA and protein levels, these cell lines produced significant amounts of MMP-9 (HL-60