Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A novel sensitive microarray approach for differential screening using probes labelled with two different

H Salin1, T Vujasinovic, A Mazurie

  • 1LGN, UMR 7091, CNRS, Bâtiment CERVI, 5ème Etage, Hôpital Pitié Salpêtrière, 83 boulevard de l'Hôpital, F-75013 Paris, France.

Nucleic Acids Research
|February 14, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Increased genomic alteration complexity and telomere shortening in B-CLL cells resistant to radiation-induced apoptosis.

Cytogenetic and genome research·2009
Same author

Loss of phase synchrony in an animal model of partial status epilepticus.

Neuroscience·2007
Same author

Search for coherent charged pion production in neutrino-carbon interactions.

Physical review letters·2005
Same author

Has adaptive dynamics contributed to the understanding of adaptive and sympatric speciation?

Journal of evolutionary biology·2005
Same author

Nicotine reinforcement and cognition restored by targeted expression of nicotinic receptors.

Nature·2005
Same author

Evidence for muon neutrino oscillation in an accelerator-based experiment.

Physical review letters·2005

This study introduces a novel microarray technique using dual radioelement labeling for efficient gene expression comparison. It enables sensitive quantification of mRNA from limited biological samples, ideal for fields like neuroscience.

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Gene expression analysis is crucial for understanding biological processes.
  • Comparing gene expression between different biological samples often requires substantial amounts of mRNA.
  • Existing microarray techniques can be limited by sample quantity and sensitivity.

Purpose of the Study:

  • To develop a novel microarray approach for differential gene expression screening.
  • To enable sensitive mRNA quantification from limited biological samples.
  • To facilitate the comparison of gene expression profiles between two distinct biological states.

Main Methods:

  • Utilized a microarray platform with probes labeled with two distinct radioelements (e.g., 3H and 35S or 33P).

Related Experiment Videos

  • Employed a MicroImager digital autoradiography system for real-time, high-resolution image acquisition.
  • Developed a data processing algorithm to separate and analyze hybridization signals specific to each radioelement.

    • Main Results:

    • Achieved simultaneous screening of gene expression from two biological samples using less than 100 ng of mRNA without amplification.
    • Demonstrated high sensitivity, quantifying as few as 10^7 molecules (control) and 2 x 10^5 molecules (endogenous tyrosine hydroxylase mRNA).
    • Successfully compared gene expression in limited sample quantities.

    Conclusions:

    • The developed double radioactive labeling microarray technique is effective for differential gene expression analysis.
    • This method is suitable for comparing gene expression in scarce biological samples.
    • The technique holds significant potential for applications in various biological research fields, including neuroscience.