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Structural analysis of the rDNA intergenic spacer of Tuber borchii
Pasquapina Ciarmela1, Lucia Potenza, Sabrina Zeppa
1Istituto di Chimica Biologica Giorgio Fornaini, Universit degli Studi di Urbino, Via A. Saffi 2, 61029 Urbino (PU), Italy.
Journal of Biomolecular Structure & Dynamics
|February 15, 2002
Abstract:
The sequence and characterisation of the entire nuclear rDNA intergenic spacer (IGS) for the genus Tuber are presented. Sequence analyses showed that the organisation of the Tuber borchii rDNA IGS is typical of rDNA spacers, consisting of a central repetitive region and flanking unique sequences on either side. Direct repeats, symmetry elements, tandem repeats and possible areas of recombination were found. The putative ends of the 25S and 17S rDNA were identified. The presence of 5S rDNA in the IGS region was excluded.