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Matrix metalloproteinases 2 and 9 in otitis media with effusion
C R Jennings1, L Guo, H M Collins
1Department of Otorhinolaryngology and Head and Neck Surgery, Queen's Medical Centre, University Hospital, Nottingham, UK.
Clinical Otolaryngology and Allied Sciences
|February 15, 2002
Summary
Matrix metalloproteinase 2 (MMP 2) and MMP 9 levels are higher in thick middle ear fluid, suggesting a role in otitis media with effusion (OME) and tympanic membrane damage.
Area of Science:
- Otolaryngology
- Biochemistry
- Pediatrics
Background:
- Otitis media with effusion (OME) is a common pediatric condition.
- The role of matrix metalloproteinases (MMPs) in OME pathogenesis is not fully understood.
Purpose of the Study:
- To investigate the presence and activity of MMP 2 and MMP 9 in OME effusions.
- To compare MMP levels in relation to effusion thickness and ventilation tube insertions.
Main Methods:
- Qualitative and quantitative analysis of MMP 2 and MMP 9 in OME effusions.
- Comparison of enzyme activity in thick versus thin effusions.
- Correlation of MMP concentrations with the number of ventilation tube sets.
Main Results:
- Both MMP 2 and MMP 9 activity were significantly higher in thick OME effusions compared to thin effusions.
- MMP 9 concentrations did not correlate with the number of ventilation tube insertions.
- MMP 2 concentrations showed a significant correlation with the number of ventilation tube insertions (ANOVA P < 0.05).
Conclusions:
- MMPs, particularly MMP 2 and MMP 9, are present in OME effusions.
- These enzymes may play a role in the pathological processes of OME, including tympanic membrane damage.
- MMP 2 levels may be indicative of the chronicity or severity of OME requiring repeated ventilation tube insertions.