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Inability to clonally expand fetal progenitors from maternal blood
Bernhard Zimmermann1, Wolfgang Holzgreve, Xiao Yan Zhong
1Department of Obstetrics and Gynaecology, University of Basel, Switzerland.
Fetal Diagnosis and Therapy
|February 15, 2002
Abstract
Objectives:
To confirm the recent report of the culture of single clones of fetal progenitor cells from maternal blood.
Methods:
Hemopoietic progenitor cells were cultured from 16 blood samples obtained from women pregnant with a male singleton fetus. Single colonies were isolated by micro-manipulation and examined by multiplex real-time PCR.
Results:
Of a total of 1,674 colonies examined, 1,648 were identified as being maternal. No colonies were detected of fetal origin.
Conclusions:
The clonal expansion of single fetal colonies from the maternal circulation is not feasible by current methods.