Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Molecular confirmation of Ewing sarcoma.

R Dagher1, T A Pham, L Sorbara

  • 1Pediatric Oncology Branch , National Cancer Institute. National Institutes of Health, Bethesda, Maryland 20892, USA.

Journal of Pediatric Hematology/Oncology
|February 16, 2002
PubMed
Summary

Reverse transcription polymerase chain reaction (RT-PCR) testing is crucial for diagnosing Ewing sarcoma and related tumors. Optimizing sample quality and biopsy techniques improves molecular marker assessment and diagnostic accuracy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>"Taking PrEP every day for me is a challenge":</i> Barriers, and facilitators to accessing HIV pre-exposure prophylaxis (PrEP) services among young people in Gauteng, South Africa.

medRxiv : the preprint server for health sciences·2026
Same author

Royal College of Radiologists Guidance Statements on the Use of Auto-contouring in Radiotherapy.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

[The overall study protocol for the West China Bone Health Cohort].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2025
Same author

[Brain activity alterations in chronic cough: a resting-state functional magnetic resonance imaging study].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2025
Same author

Evaluation of Radiotherapy Plan Robustness: A Systematic Literature Review.

Clinical oncology (Royal College of Radiologists (Great Britain))·2025
Same author

Designing AI-powered healthcare assistants to effectively reach vulnerable populations with health care services: A discrete choice experiment among South African university students.

medRxiv : the preprint server for health sciences·2025

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Ewing sarcoma and primitive neuroectodermal tumors (PNET) are aggressive cancers requiring accurate molecular diagnosis.
  • Reverse transcription polymerase chain reaction (RT-PCR) is a key technique for detecting characteristic translocations in these tumors.

Purpose of the Study:

  • To retrospectively analyze RT-PCR results and demographic data in patients with suspected Ewing sarcoma/PNET.
  • To identify factors influencing molecular marker status determination.

Main Methods:

  • Retrospective analysis of tumor samples from 76 patients diagnosed with Ewing sarcoma/PNET.
  • Histopathologic review and RT-PCR testing for translocations (EWS-FLI, EWS-ERG).
  • Evaluation of biopsy methods and sample quality.

Related Experiment Videos

Main Results:

  • RT-PCR confirmed translocations in 58 patients, including EWS-FLI and EWS-ERG types.
  • Nested RT-PCR identified translocations in four additional cases.
  • Inadequate samples or alternative diagnoses were noted in 18 translocation-negative cases.

Conclusions:

  • Translocation-negative cases warrant investigation for alternative diagnoses, need for nested RT-PCR, or inadequate samples.
  • Open biopsy is recommended over needle biopsy to improve diagnostic accuracy and avoid repeat procedures.