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Avoiding cross hybridization by choosing nonredundant targets on cDNA arrays
1Center for Biological Sequence Analysis, BioCentrum-DTU, Technical University of Denmark, Building 208, 2800 Lyngby, Denmark. hbjorn@cbs.dtu.dk
Bioinformatics (Oxford, England)
|February 16, 2002
Abstract:
PROBEWIZ designs PCR primers for amplifying probes for cDNA arrays. The probes are designed to have minimal homology to other expressed sequences from a given organism. The primer selection is based on user-defined penalties for homology, primer quality, and proximity to the 3' end.