Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Breaking the singleton of germination protease.

Jimin Pei1, Nick V Grishin

  • 1Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9050, USA.

Protein Science : a Publication of the Protein Society
|February 16, 2002
PubMed
Summary

Germination protease (GPR) is homologous to hydrogenase maturation protease (HybD), suggesting a shared metal-binding mechanism essential for spore germination and protease function. This research clarifies GPR

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Butterfly taxa discovery via genomic analysis.

The taxonomic report of the International Lepidoptera Survey·2026
Same author

<b>Revisiting <i>Colobura</i> (Lepidoptera: Nymphalidae): Using integrative taxonomy to identify a new species, <i>C. cryptica</i> sp. nov., and revise geographic boundaries</b>.

Zootaxa·2026
Same author

Structural insights into predicted protein-protein interactions and protein complexes in the human proteome.

Scientific reports·2026
Same author

Universal and Lineage-Specific Patterns in the Distribution of ECOD Domain Homology Groups Across Superkingdoms.

Proteins·2026
Same author

Family-level specialization in protein domain insertion architectures.

Protein science : a publication of the Protein Society·2026
Same author

A taxonomic overview of the <i>Apodemia mormo</i> complex from a genomic perspective.

The taxonomic report of the International Lepidoptera Survey·2026

Area of Science:

  • Microbiology
  • Structural Biology
  • Biochemistry

Background:

  • Germination protease (GPR) is crucial for Bacillus and Clostridium spore germination.
  • The active site and catalytic mechanisms of GPRs remain largely unknown.
  • Recent 3-D structure determination of Bacillus megaterium GPR offers new insights.

Purpose of the Study:

  • To elucidate the structural and functional relationship of GPRs with other proteins.
  • To identify conserved motifs and potential active site residues in GPRs.
  • To understand the catalytic mechanisms of GPRs and related proteases.

Main Methods:

  • Comparative structural analysis of GPRs.
  • Sequence analysis of GPRs and homologous proteins.
  • Identification of conserved motifs and residues.

Related Experiment Videos

Main Results:

  • GPR is homologous to bacterial hydrogenase maturation protease (HybD).
  • Two conserved motifs and two acidic residues are shared among GPRs, HybD, and hypothetical proteins.
  • Metal ion binding is indicated as crucial for the function of this protease superfamily.

Conclusions:

  • GPR likely functions as a metal-regulated protease.
  • The study localizes the active site of GPRs.
  • Insights into the catalytic mechanisms of a superfamily of metal-regulated proteases are provided.