Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Capturing chromosome conformation.

Job Dekker1, Karsten Rippe, Martijn Dekker

  • 1Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA. jdekker@fas.harvard.edu

Science (New York, N.Y.)
|February 16, 2002
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

UnionLoops: a workflow for calling chromatin loops across related Hi-C datasets with improved specificity, precision, and sensitivity.

Genome biology·2026
Same author

Mutant IDH1 blocks neutropoiesis by repressing myeloid progenitor programs.

Blood·2026
Same author

Dissecting the cellular architecture of breast cancer brain metastases reveals prognostically distinct immune landscapes.

Cancer cell·2026
Same author

Faulted ZnS:Mn,Cu Nanorods Provide Nanoscale Mechanoluminescence Excitation in a Biologically Relevant Force Range.

ACS nano·2026
Same author

Fiber intake associates with increased treatment response in patients with multiple myeloma along with changes in gut microbiome.

Blood advances·2026
Same author

UnionLoops: a workflow for calling chromatin loops across related Hi-C datasets with improved specificity, precision, and sensitivity.

bioRxiv : the preprint server for biology·2026
Same journal

A native sulfur deposit in Gale crater, Mars.

Science (New York, N.Y.)·2026
Same journal

Coordinated demise of harmful algal blooms.

Science (New York, N.Y.)·2026
Same journal

Genetic effects put into context.

Science (New York, N.Y.)·2026
Same journal

Bacteria share proteins to survive antibiotics.

Science (New York, N.Y.)·2026
Same journal

Impacts shaped Earth's first continents.

Science (New York, N.Y.)·2026
Same journal

Erratum for the Report "Covalently bonded single-molecule junctions with stable and reversible photoswitched conductivity" by C. Jia <i>et al</i>.

Science (New York, N.Y.)·2026
See all related articles

This study introduces a new method to map genomic interactions, revealing chromatin fiber flexibility and 3D genome organization. The approach visualizes chromosome structures in yeast, offering insights into genome spatial arrangement.

Area of Science:

  • Genomics
  • Molecular Biology
  • Biophysics

Background:

  • Understanding the three-dimensional (3D) organization of genomes within the nucleus is crucial for deciphering gene regulation and cellular function.
  • Existing methods provide insights into genome architecture but often lack the resolution to capture dynamic changes or detailed structural properties of chromatin fibers.

Purpose of the Study:

  • To develop and validate a novel methodology for quantifying interaction frequencies between genomic loci.
  • To apply this method to study the spatial organization and physical properties of chromatin in yeast (Saccharomyces cerevisiae).
  • To generate a population-average 3D model of a specific chromosome and investigate its structural dynamics.

Main Methods:

  • Generation of an interaction frequency matrix between genomic sites.

Related Experiment Videos

  • Application of the methodology to the yeast Saccharomyces cerevisiae, including during meiosis and at the G1 stage of the cell cycle.
  • Analysis of chromatin flexibility and conformational differences between AT- and GC-rich domains.

    • Main Results:

    • The developed approach successfully maps interaction frequencies, revealing the relative spatial disposition of genomic loci.
    • Known features of yeast nuclear organization and dynamic changes during meiosis were confirmed.
    • Chromatin was found to be highly flexible, with distinct conformations observed for AT- and GC-rich domains.
    • A population-average 3D model of yeast chromosome III was determined, depicting it as a contorted ring.

    Conclusions:

    • The described methodology provides a powerful tool for analyzing genome-wide spatial organization and chromatin properties across diverse organisms.
    • The study highlights the dynamic and flexible nature of chromatin, with distinct structural behaviors of different genomic domains.
    • The 3D model of chromosome III offers a detailed view of its organization within the yeast nucleus.